The OCTOUS facility collaborates with research groups internationally as well as in the UK and internally within STFC to develop and support leading research facilities and research programs.
Recent highlights include:
article in Angewante Chemie describes new live cell probes with long
fluorescence lifetimes (>160 -1000 ns) when bound to DNA and shorter
lifetimes in the cytoplasm.
The principal advantages of metal complexes in bio-imaging are long emission lifetimes (>10-7 sec) and increased photostability. This combined with technological advances in optics and electronics has resulted in the emergence of microsecond time-resolved techniques, namely; Phosphorescence Lifetime Imaging Microscopy (PLIM) and Time-Resolved Emission Imaging Microscopy (TREM). The lifetimes are orders of magnitude greater than that of background “autofluorescence” in the cell and tissues and allow previously unattainable levels of sensitivity. Their suitability for imaging deep within tissue is under investigation.
Prof Stan Botchway (CLF contact)
Publication: Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA. Baggaley E, Gill MR, Green NH, Turton D, Sazanovich IV, Botchway SW, Smythe C, Haycock JW, Weinstein JA, Thomas JA. Angew Chem Int Ed Engl. 2014 Mar 24;53(13):3367-71. doi: 10.1002/anie.201309427.
A recent cover article in ChemComm showing drug behaviour models in the human respiratory system
Using the Octopus laser imaging facility at the Science and
Technology Facilities Council (STFC) Central Laser Facility, the scientists
trapped individual solid particles of the drug salbutamol sulphate. They
suspended them in air to test how they behave in conditions modelled to
simulate those in the human respiratory system.
Over 73 million inhalers are used every year in the UK. By
studying how the expelled drug particles might behave as they enter the human
respiratory tract and travel into the lungs, this new research could lead to an
improvement in the formulation of these drug delivery systems, increasing their
effectiveness whilst reducing negative side effects. To overcome the natural defence mechanisms of
the body, complex delivery devices and extremely small drug particles are
required.These particles are typically
2-5 micron in diameter.Any moisture that
clings to the particles as they travel from inhaler to lung is likely to
increase the particles’ size, and this may affect the site of particle
deposition within the lung.It can
result in the drug being deposited in a non-ideal site, giving rise to less
effective treatment and potentially an increase in side effects.
The research could lead to more efficient inhalers to
deliver drugs for respiratory problems. Our results will also inform
pharmaceutical companies who may be looking to improve the chemical structure
of the drug, increasing effectiveness whilst reducing negative side effects.
Dr Andy Ward (CLF Contact)
The research team comprised scientists from the Universities
of Birmingham and Cambridge, Imperial College London and the STFC Central Laser
Facility, based at the Research Complex at Harwell, Oxfordshire. The research
was funded by STFC, NERC and the European Research Council.
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