CLF Symposium and Workshop on correlative light, electron, and X-ray microscopy

About the symposium

The symposium consists of lectures from world-leading scientists working in the area of correlative microscopy with light, electrons, and X-rays. The aim is to inform the community of the latest developments in correlative microscopy, and for the community to discuss and feed back its requirements for correlative microscopy development and provision in the UK.

About the workshop

The symposium will be followed by a 3 day workshop dedicated to demonstrations and practical training sessions in correlative microscopy techniques. Correlative microscopy workflows to be demonstrated include cryo-TEM, cryo-X-ray microscopy, cryo-fluorescence and super-resolution microscopy, and sample marking using multiphoton techniques. The final day of the workshop will be dedicated to data analysis techniques.

Registration is now open, however all of the places for the workshops are now taken. Space is limited and in the event of oversubscription delegates will be selected to ensure a good balance of research areas and institutions. A dinner will be held on site on the evening of Monday 6 March (fee applies).

Symposium only (£25) includes:

Lunch on Monday and Tuesday

Morning and afternoon refreshments

About the venue

The Harwell campus is the site of the UK's large scale science facilities. Located about 8 miles south of Oxford, it houses the Diamond Light Source synchrotron facility, the Central Laser Facility (CLF), and the ISIS pulsed neutron source. Imaging facilities available include the Electron Bio-Imaging Centre (eBIC) at Diamond Light Source, optical microscopy at the CLF's Octopus facility in the Research Complex at Harwell, and scanning and full-field X-ray microscopy beamlines at Diamond.

Click here for a map and some details about transport.

Programme

6th March 2017

10:00	Arrival and registration
11:00	Welcoming remarks
11:15	Invited talk	CLEM: 1+1=3	Paul Verkade (Bristol University)
11:45	Keynote lecture	Light, electrons, ions: Correlative approaches to understanding life on the early Earth	Martin Saunders (University of Western Australia)
12:45	Lunch
13:45	Invited talk	Ultrastructural analysis of in vivo imaged synapses	Graham Knott (École polytechnique fédérale de Lausanne)
14:15	Invited talk	Corrrelative Tomography: Multiscale, Multifaceted and Time-lapse Imaging in 3D	Phillip Withers (Manchester University)
14:45	Invited talk	Correlative microscopy of oxide nanoparticles at biological barriers	Alex Porter (Imperial College London)
15:15	Invited talk	Super-resolution CLEM with optimal structural preservation	Rainer Kaufman (Oxford University)
15:45	Coffee break
16:15	Keynote lecture	An array tomographic approach to the study of axons and synapses in the brain	Kristina Micheva (Stanford University)
17:15	Invited talk	Correlative in-resin super-resolution and electron microscopy in culture cells and tissue	Errin Johnson (Oxford University)
17:45	Poster session
19:15	Dinner

7th March 2017

09:00	Keynote lecture	Correlative microscopy for structural cell biology: Studying the architecture of cellular membranes	Wanda Kukulski (Cambridge University)
10:00	Invited talk	Flexible multidimensional registration software for correlative microscopies with refined accuracy mapping: eC-CLEM easy Cell Correlative Light to Electron Microscopy	Perrine Paul-Gilloteaux (CNRS, Nantes)
10:30	Coffee break
11:00	Invited talk	Multiscale correlative imaging using light, X-rays and electrons	Lucy Collinson (Crick Institute)
11:30	Invited talk	TBA	Michele Darrow (Diamond Light Source)
12:00	Invited talk	Visualizing HIV infection using automated cryoCLEM	Peijun Zhang (eBIC, Diamond Light Source)
12:30	Lunch
13:30	Keynote lecture	Exploring the potential of the FIB-SEM in CLEM. Automation and targeting	Anna Steyer (EMBL Heidelberg)
14:30	Discussion session
15:30	Coffee break and facility tours
17:00	Departure	For delegates attending symposium only

Workshop

8th-10th March 2017

Practicals to demonstrate experimental aspects of correlative microscopy. Participants will be able to watch as samples are prepared and data are collected with their chosen technique. Depending on the demonstrations/experimental stream chosen there will be more or less chance of hands on practice. Workflows to be deminstrated are expected to include:

Cryo-EM
Super Resolution and cryo-fluorescence microscopy
Cryo-X-ray microscopy
Sample fiducial marking using multiphoton methods
Correlative microscopy data analysis

Accommodation

Accommodation is available at a number of local hotels. Some are listed below. Availability of accommodation in the area is quite limited so early booking is strongly recommended. If you require any assistance with transport from your accommodation to the symposium/workshop venue, please contact the organisers.