Chromosomes can be thought of as USBs that contain the vast majority of our genetic information in the form of DNA. The discovery of chromosomes and the structural elements that lay within them can be credited in part to the advancement in microscopy. However, even with the advancement in the technique, it is extremely challenging to get a visual representation of elements like chromatin organisation, which facilitates chromosome formation.
Understanding the structural basis of chromosome and chromatin is crucial in providing insights about disease states in cells. Researchers from Aga Khan University, Brookhaven National Lab worked alongside CLF scientist, Prof Stan Botchway, have reviewed different microscopy techniques that are alternatives to traditional X-ray and cryo-electron microscopy. This follows extensive work on chromosomes and advanced imaging by the team over many years.
In their review, the researchers highlighted that fluorescence lifetime imaging microscopy (FLIM) and super-resolution microscopy (SRM) can be viable options in live-cell imaging of chromosomes and chromatin. Prof Stan Botchway goes on to add that, “combining these two techniques is seen as greater than the sum of their parts for chromosome imaging and research."
The paper can be found at DOI: 10.1007/s10577-021-09654-5