Life and Science imaging
25 Nov 2016
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Laser-based imaging techniques are used across the CLF for applications spanning life sciences and engineering.

Yes

​​​​​​​​​Dr Anne Osterrieder from Oxford Brookes University investigating the plant secretoryome using the LSF (2011)

 

​The microscopy techniques of the OCTOPUS facility allow incredibly precise measurements of biological samples. The microscopes are able to detect even single molecules fluorescing and can image molecular processes within cells to resolutions of tens of nanometres.​


Examples of Recent Publications​


Metabolons on the Plant ER (2018)​​​

Kriechbaumer V., Botchway S.W. (2018) Metabolons on the Plant ER. In: Hawes C., Kriechbaumer V. (eds) The Plant Endoplasmic Reticulum. Methods in Molecular Biology, vol 1691. Humana Press, New York, NY


Multimodal super-resolution optical microscopy using a transition metalbased probe provides unprecedented capabilities for imaging both nuclear chromatin and mitochondria (2017)​​​

Multimodal super-resolution optical microscopy using a transition metal-based probe provides unprecedented capabilities for imaging both nucle-ar chromatin and mitochondria.

Sreejesh Sreedharan, Martin Gill, Esther Garcia, Hiwa K. Saeed, Darren Robinson, Aisling Byrne, Ashley James Cadby, Tia E. Keyes, Carl G W Smythe, Patrina Pellett, Jorge Bernardino de la Serna, and Jim Antony Thomas

Journal of the American Chemical Society Just Accepted Manuscript

DOI: 10.1021/jacs.7b08772 ​


Super-Resolution Nanoscopy Imaging Applied to DNA Double-Strand Breaks (2017)​​​

Sofia D'Abrantes, Sarah Gratton, Pamela Reynolds, Verena Kriechbaumer, Joseph McKenna, Stephen Barnard, Dave Clarke, and Stanley W. Botchway (2017) Super-Resolution Nanoscopy Imaging Applied to DNA Double-Strand Breaks. Radiation Research In-Press.



Fluorescence-Lifetime Imaging and Super-Resolution Microscopies Shed Light on the Directed- and Self-Assembly of Functional Porphyrins onto Carbon Nanotubes and Flat Surfaces (2017)​​

Fluorescence-Lifetime Imaging and Super-Resolution Microscopies Shed Light on the Directed- and Self-Assembly of Functional Porphyrins onto Carbon Nanotubes and Flat Surfaces B. Mao, D. G. Calatayud, V. Mirabello, N. Kuganathan, H. Ge, R. M. J. Jacobs, A. M. Shepherd, J. A. Ribeiro Martins, J. Bernardino De La Serna, B. J. Hodges, S. W. Botchway, S. I. Pascu, Chem. Eur. J. 201723, 9772.

 

Quantifying Secondary Structure Changes in Calmodulin Using 2D-IR Spectroscopy (2017)​

Quantifying Secondary Structure Changes in Calmodulin Using 2D-IR Spectroscopy

Lucy Minnes, Daniel J. Shaw, Benjamin P. Cossins, Paul M. Donaldson, Gregory M. Greetham, Michael Towrie, Anthony W. Parker, Matthew J. Baker, Alistair J. Henry, Richard J. Taylor, and Neil T. Hunt

Analytical Chemistry 2017 89 (20), 10898-10906

DOI: 10.1021/acs.analchem.7b02610 ​


Localisation and interactions between Arabidopsis auxin biosynthetic enzymes in the TAA/YUC-dependent pathway (2016)
Verena Kriechbaumer, Stanley W Botchway, Chris Hawes; Localization and interactions between Arabidopsis auxin biosynthetic enzymes in the TAA/YUC-dependent pathwayJournal of Experimental Botany, Volume 68, Issue 12, 1 June 2017, Pages 4195–4207, https://doi.org/10.1093/jxb/erw195


Direct observation by time-resolved infrared spectroscopy of the bright and the dark excited states of the [Ru(phen)2(dppz0] 2+ light-switch compound in solution and when bound to DNA (2016)
Poynton, F.E., Hall, J.P., Keane, P.M., Schwarz, C., Sazanovich, I.V., Towrie, M., Gunnlaugsson, T., Cardin, C.J., Cardin, D.J., Quinn, S.J. and Long, C., 2016. Direct observation by time-resolved infrared spectroscopy of the bright and the dark excited states of the [Ru (phen) 2 (dppz)] 2+ light-switch compound in solution and when bound to DNA. Chemical Science7(5), pp.3075-3084. ​


Enan​tiomeric Conformation Controls Rate and Yield of Photoinduced Electron Transfer in DNA Sensitized by Ru(II) Dipyridophenazine Complexes (2015)

Keane, P.M., Poynton, F.E., Hall, J.P., Clark, I.P., Sazanovich, I.V., Towrie, M., Gunnlaugsson, T., Quinn, S.J., Cardin, C.J. and Kelly, J.M., 2015. Enantiomeric conformation controls rate and yield of photoinduced electron transfer in DNA sensitized by Ru (II) Dipyridophenazine complexes. Journal of Physical Chemistry Letters6(4), pp.734-738. ​











Contact: Martin-Fernandez, Marisa (STFC,RAL,CLF)