Lightsheet microscopy
10 Jul 2019
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About

​For further information please contact Dr. Alessia Candeo.

Specifications

Technique:

Light sheet fluorescence microscopy on Leica SP8

 

Additional techniques:

Confocal microscopy

Multiphoton (MP) and non-linear microscopy

Fluorescence lifetime imaging (FLIM)

MP FLIM

FCS

 

Applications:

Biological and medical research

Developmental biology

Real time live imaging

Large samples – spheroids, tissues, small embryos

Lasers:

White light laser (80-10MHz) 470-680 nm free choice

405 nm CW

Ti:Sapph for MP 80 MHz 680-1300 nm free choice

 

Detectors:

Hamamatsu FLASH 4 Camera,

2 internal photon counting HyD, 2 internal PMTs, 2 external cooled HyD, 2 external PMTs, trans-PMT

 

Objectives:

Light sheet detection: 5x W, 10x W, 25x W

Confocal and multiphoton: 63X W, 63X Oil, 40X W, 25X IR W, 10X, 5X

 

Extra information:

Leica TCS SP8 DLS


Contact: Bateman, Benji (STFC,RAL,CLF)