Multimodality Optical NanoScopy TERminus (MONSTER)
10 Jul 2019
No
-  

 

 

 

No





 

About

The MONSTER microscope is a combined ZEISS LSM880-Elyra PS1 system in which simultaneous multi-colour imaging is enabled thanks to the dual EMCCD camera setting.​ It incorporates multiple imaging modalities including Confocal, AiryScan, Structured Illumination Microscopy and Single Molecule Localisation Microsopy (PALM/STORM). Please contact Dr. Lin Wang​ for further details.

Example data​


​​
(left) Simultaneous two-colour SIM imaging of CHO cells expressing alpha-1 an​titrypsin-TMR (red) and ER membrane-GFP (blue). Frame rate 0.6 fps (10X speed up in t​he video), resolution in SIM ~100 nm. Sample courtesy Joe Chambers at Uni of Cambridge.
(right) ​​​Correlative imaging of confocal bright-field and fluorescence images of HEK293 cells expressing mTor-mCherry. Sample courte​​​sy of Abdullah Ahmed.

Microscope specifications

​Technique Confocal Airyscan   SIM SMLM
(PALM/STORM)
 Laser wavelengths (nm)405, 458, 488, 514, 561, 633 405, 458, 488, 514, 561, 633405, 488, 561, 642405, 488, 561, 642
Simultaneous dual colour imagingYes
NoYesYes
Compatible objective lensesC-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)C-Apo 40x/1.20 w, Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)Plan Apo 63x/1.40 (oil), Plan Apo 100x/1.46 (oil)
Field of View (‎μm)135×135 (63x/1.40 NA objective)135×135 (63x/1.40 NA objective, Fast mode)80×80 (63x/1.40 NA objective)14×14 (100x/1.46 NA objective, µHP mode)/26x26 (HP mode)
 Lateral resolution (nm) (λ=488nm)240 (63x/1.40 NA objective)140(normal mode)/120(2D SR or 3D mode)/145 in X, 180 in Y (Fast SR mode)12020 (localisation precision)
 Axial resolution (nm) (λ=488nm)700 (63x/1.40 NA objective)350 (normal mode)/ 450 (Fast SR mode)30050
One shot 3D methodN/AN/AN/Aphase ramp, 1µm depth
Sample thickness (‎μm)1001002010
 Temporal resolution0.85 fps at 480x480 pixels 27 fps at 480x480 pixels in Fast mode1.6 second per frame (at 24 ms integration time)several minutes



​​



Contact: Wang, Lin (STFC,RAL,CLF)