Symposium and Workshop on correlative light, electron, and X-ray microscopy
24 Jan 2017
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​​About the symposium

The symposium consists of lectures from world-leading scientists working in the area of correlative microscopy with light, electrons, and X-rays. The aim is to inform the community of the latest developments in correlative microscopy, and for the community to discuss and feed back its requirements for correlative microscopy development and provision in the UK.

About the workshop

The symposium will be followed by a 3 day workshop dedicated to demonstrations and practical training sessions in correlative microscopy techniques. Correlative microscopy workflows to be demonstrated include cryo-TEM, cryo-X-ray microscopy, cryo-fluorescence and super-resolution microscopy, and sample marking using multiphoton techniques. The final day of the workshop will be dedicated to data analysis techniques.

Registration is now open, however all of the places for the workshops are now taken. Space is limited and in the event of oversubscription delegates will be selected to ensure a good balance of research areas and institutions. A dinner will be held on site on the evening of Monday 6 March (fee applies).

Symposium only (£25) includes:

Lunch on Monday and Tuesday

Morning and afternoon refreshments

About the venue

The Harwell campus is the site of the UK's large scale science facilities. Located about 8 miles south of Oxford, it houses the Diamond Light Source synchrotron facility, the Central Laser Facility (CLF), and the ISIS pulsed neutron source. Imaging facilities available include the Electron Bio-Imaging Centre (eBIC) at Diamond Light Source, optical microscopy at the CLF's Octopus facility in the Research Complex at Harwell, and scanning and full-field X-ray microscopy beamlines at Diamond.

Click here for a map and some details about transport.​

Programme

6th March 2017

10:00
Arrival and registration
  
11:00​
Welcoming remarks 
 
11:15Invited talkCLEM: 1+1=3Paul Verkade (Bristol University)
11:45Keynote lectureLight, electrons, ions: Correlative approaches to understanding life on the early EarthMartin Saunders (University of Western Australia)
12:45Lunch  
13:45Invited talkUltrastructural analysis of in vivo imaged synapsesGraham Knott (École polytechnique fédérale de Lausanne)
14:15
Invited talkCorrrelative Tomography: Multiscale, Multifaceted and Time-lapse Imaging in 3DPhillip Withers (Manchester University)
14:45Invited talkCorrelative microscopy of oxide nanoparticles at biological barriersAlex Porter (Imperial College London)
15:15Invited talkSuper-resolution CLEM with optimal structural preservationRainer Kaufman (Oxford University)
15:45Coffee break  
16:15Keynote lectureAn array tomographic approach to the study of axons and synapses in the brainKristina Micheva (Stanford University)
17:15Invited talkCorrelative in-resin super-resolution and electron microscopy in culture cells and tissueErrin Johnson (Oxford University)
17:45Poster session  
19:15Dinner  

7th March 2017

​09:00
Keynote lecture
Correlative microscopy for structural cell biology: Studying the architecture of cellular membranes
Wanda Kukulski (Cambridge University)
10:00Invited talkFlexible multidimensional registration software for correlative microscopies with refined accuracy mapping: eC-CLEM easy Cell Correlative Light to Electron MicroscopyPerrine Paul-Gilloteaux (CNRS, Nantes)
10:30Coffee break  
11:00Invited talkMultiscale correlative imaging using light, X-rays and electronsLucy Collinson (Crick Institute)
11:30Invited talkTBAMichele Darrow (Diamond Light Source)
12:00Invited talkVisualizing HIV infection using automated cryoCLEMPeijun Zhang (eBIC, Diamond Light Source)
12:30Lunch  
13:30Keynote lectureExploring the potential of the FIB-SEM in CLEM. Automation and targetingAnna Steyer (EMBL Heidelberg)
14:30Discussion session  
15:30Coffee break and facility tours  
17:00DepartureFor delegates attending symposium only 

 

Workshop

8th-10th March 2017

Practicals to demonstrate experimental aspects of correlative microscopy. Participants will be able to watch as samples are prepared and data are collected with their chosen technique. Depending on the demonstrations/experimental stream chosen there will be more or less chance of hands on practice. Workflows to be deminstrated are expected to include:

  • Cryo-EM
  • Super Resolution and cryo-fluorescence microscopy
  • Cryo-X-ray microscopy
  • Sample fiducial marking using multiphoton methods
  • Correlative microscopy data analysis

​Accommodation

Accommodation is available at a number of local hotels. Some are listed below. Availability of accommodation in the area is quite limited so early booking is strongly recommended. If you require any assistance with transport from your accommodation to the symposium/workshop venue, please contact the organisers.

Local Hotel or B&B Accommodation

Within 5 miles of Harwell Campus

Premier Inn Hotel Milton Interchange

Milton Hill House Hotel

Steventon House Hotel

Cherry Tree B&B, Steventon

The Kingswell Hotel, Harwell

Greensands B&B

Abingdon area (approx. 9 miles from Harwell Campus)

Oxford Abingdon Hotel

Premier Inn Hotel, Abingdon

Crown & Thistle Hotel, Abingdon

Kingfisher Barns B&B Abingdon

The Dog House, Frilford Heath

B&B Rafters, Marcham

​Didcot (approx. 5 miles from Harwell Campus, within 2 miles of Didcot Parkway Station)

Penny's B&B, Didcot

The House on the Corner B&B, Didcot

Southern Wood B&B, Didcot

​Newbury area (approx. 14 miles from Harwell Campus)

Donnington Valley Hotel Newbury

Hilton Hotel Newbury North

Elcot Mercure Hotel

For any enquiries please contact Heather Watts (01235 445001, heather.watts@stfc.ac.uk)

Organisers

David Clarke (STFC Central Laser Facility, Research Complex at Harwell)

Lucy Collinson (Francis Crick Institute, London)

Elizabeth Duke (Diamond Light Source)

Marisa Martin-Fernandez (STFC Central Laser Facility, Research Complex at Harwell)

Paul Verkade (University of Bristol)

Sponsors

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Contact: Bateman, Benji (STFC,RAL,CLF)