Connor R. Barker, Megan L. Poole, Matthew Wilkinson, James Morison, Alan Wilson, Gina Little,,Edward J. Stuckey, Rebecca J. L. Welbourn, Andrew D. Ward and Martin D. King
Dr. Hiwa K. Saeed, Dr. Paul J. Jarman, Dr. Sreejesh Sreedharan, Dr. Rachel Mowll, Alexander J. Auty, Adrien A. P. Chauvet, Prof. Carl G. W. Smythe, Dr. Jorge Bernardino de la Serna, Prof. Jim A. Thomas
David T Hinds, Samir Adam Belhout, Paula E. Colavita, Andrew D. Ward, Susan J Quinn
Tara A. Eastwood, Karen Baker, Bree R. Streather, Nyasha Allen, Lin Wang, Stanley W. Botchway, Ian R. Brown, Jennifer R. Hiscock, Christopher Lennon, Daniel P. Mulvihill
2020
The reaction of oleic acid monolayers with gas-phase ozone at the air–water interface: the effect of sub-phase viscosity, and inert secondary components
Martin D. King; Stephanie H. Jones; Claire O. M. Lucas; Katherine C. Thompson; Adrian R. Rennie; Andrew D. Ward; Amelia A. Marks; Fleur N. Fisher; Christian Pfrang; Arwel V. Hughes; Richard A. Campbell
Physical Chemistry Chemical Physics, 22, 28032–28044 (2020), DOI: 10.1039/D0CP03934A.
SARS-CoV-2 and mitochondrial health: implications of lifestyle and ageing
Alistair V. W. Nunn; Geoffrey W. Guy; Wolfgang Brysch; Stanley W. Botchway; Wayne Frasch; Edward J. Calabrese; Jimmy D. Bell
Immunity & Ageing, 17(1), 33 (2020), DOI: 10.1186/s12979-020-00204-x.
Mechanisms of Action of EGFR Tyrosine Kinase Receptor Incorporated in Extracellular Vesicles
Laura C. Zanetti-Domingues; Scott E. Bonner; Marisa L. Martin-Fernandez; Valentina Huber
Cells, 9(11), 2505 (2020), DOI: 10.3390/cells9112505.
Cooperation and Interplay between EGFR Signalling and Extracellular Vesicle Biogenesis in Cancer
Laura C. Zanetti-Domingues; Scott E. Bonner; R. Sumanth Iyer; Marisa L. Martin-Fernandez; Valentina Huber
Cells, 9(12), 2639 (2020), DOI: 10.3390/cells9122639.
Directly imaging the localisation and photosensitization properties of the pan-mTOR inhibitor, AZD2014, in living cancer cells
Abdullah R. Ahmed; Alessia Candeo; Sofia D'Abrantes; Sarah R. Needham; Rahul B. Yadav; Stanley W. Botchway; Anthony W. Parker
Journal of Photochemistry and Photobiology B: Biology, 213, 112055 (2020), DOI: 10.1016/j.jphotobiol.2020.112055.
Using Mie Scattering to Determine the Wavelength-Dependent Refractive Index of Polystyrene Beads with Changing Temperature
Megan R. McGrory; Martin D. King; Andrew D. Ward
The Journal of Physical Chemistry A, 124(46), 9617–9625 (2020), DOI: 10.1021/acs.jpca.0c06121.
The smfBox is an open-source platform for single-molecule FRET
Benjamin Ambrose; James M. Baxter; John Cully; Matthew Willmott; Elliot M. Steele; Benji C. Bateman; Marisa L. Martin-Fernandez; Ashley Cadby; Jonathan Shewring; Marleen Aaldering; Timothy D. Craggs
Nature Communications, 11(1), 5641 (2020), DOI: 10.1038/s41467-020-19468-4.
Super-Resolution Fluorescence Microscopy Reveals Clustering Behaviour of Chlamydia pneumoniae’s Major Outer Membrane Protein
Amy E. Danson; Alex McStea; Lin Wang; Alice Y. Pollitt; Marisa L. Martin-Fernandez; Isabel Moraes; Martin A. Walsh; Sheila MacIntyre; Kimberly A. Watson
Biology, 9(10), 344 (2020), DOI: 10.3390/biology9100344.
Porous Carbon Microparticles as Vehicles for the Intracellular Delivery of Molecules
Luis M. Magno; David T. Hinds; Paul Duffy; Rahul B. Yadav; Andrew D. Ward; Stanley W. Botchway; Paula E. Colavita; Susan J. Quinn
Frontiers in Chemistry, 8, 576175 (2020), DOI: 10.3389/fchem.2020.576175.
A small molecule inhibitor of HER3: a proof-of-concept study
Audrey Colomba; Martina Fitzek; Roger George; Gregory Weitsman; Selene K. Roberts; Laura C. Zanetti-Domingues; Michael Hirsch; Daniel J. Rolfe; Sana Mehmood; Andrew Madin; Jeroen Claus; Svend Kjaer; Ambrosius P. Snijders; Tony Ng; Marisa L. Martin-Fernandez; David M. Smith; Peter J. Parker
Biochemical Journal, 477(17), 3329–3347 (2020), DOI: 10.1042/BCJ20200496.
Measurement of the fluorescence lifetime of GFP in high refractive index levitated droplets using FLIM
Nick M. Davidson; Peter J. Gallimore; Benji C. Bateman; Andrew D. Ward; Stanley W. Botchway; Markus Kalberer; Marina K. Kuimova; Francis D. Pope
Physical Chemistry Chemical Physics, 22(26), 14704–14711 (2020), DOI: 10.1039/C9CP06395A.
The Photophysical Properties of Triisopropylsilyl-ethynylpentacene—A Molecule with an Unusually Large Singlet-Triplet Energy Gap—In Solution and Solid Phases
Fabio A. Schaberle; Carlos Serpa; Luis G. Arnaut; Andrew D. Ward; Joshua K. G. Karlsson; Alparslan Atahan; Anthony Harriman
Chemistry, 2(2), 545–564 (2020), DOI: 10.3390/chemistry2020033.
A Dinuclear Ruthenium(II) Complex Excited by Near-Infrared Light through Two-Photon Absorption Induces Phototoxicity Deep within Hypoxic Regions of Melanoma Cancer Spheroids
Ahtasham Raza; Stuart A. Archer; Simon D. Fairbanks; Kirsty L. Smitten; Stanley W. Botchway; James A. Thomas; Sheila MacNeil; John W. Haycock
Journal of the American Chemical Society, 142(10), 4639–4647 (2020), DOI: 10.1021/jacs.9b11313.
From sunscreens to medicines: Can a dissipation hypothesis explain the beneficial aspects of many plant compounds?
Alistair V. W. Nunn; Geoffrey W. Guy; Stanley W. Botchway; Jimmy D. Bell
Phytotherapy Research, 34(8), 1868–1888 (2020), DOI: 10.1002/ptr.6654.
Miro2 tethers the ER to mitochondria to promote mitochondrial fusion in tobacco leaf epidermal cells
Rhiannon R. White; Congping Lin; Ian Leaves; Inês G. Castro; Jeremy Metz; Benji C. Bateman; Stanley W. Botchway; Andrew D. Ward; Peter Ashwin; Imogen Sparkes
Communications Biology, 3(1), 161 (2020), DOI: 10.1038/s42003-020-0872-x.
Supramolecular clustering of the cardiac sodium channel Nav1.5 in HEK293F cells, with and without the auxiliary β3-subunit
Samantha C. Salvage; Johanna S. Rees; Alexandra McStea; Michael Hirsch; Lin Wang; Christopher J. Tynan; Matthew W. Reed; Jennifer R. Irons; Richard Butler; Andrew J. Thompson; Marisa L. Martin-Fernandez; Christopher L. H. Huang; Antony P. Jackson
The FASEB Journal, 34(3), 3537–3553 (2020), DOI: 10.1096/fj.201701473RR.
Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function
Natalia Fili; Yukti Hari-Gupta; Bjork Aston; Ália dos Santos; Rosemarie E. Gough; Bana Alamad; Lin Wang; Marisa L. Martin-Fernandez; Christopher P. Toseland
Journal of Biological Chemistry, 295(2), 337–347 (2020), DOI: 10.1074/jbc.RA119.010142.
The development of a high throughput drug-responsive model of white adipose tissue comprising adipogenic 3T3-L1 cells in a 3D matrix
Alexander D. Graham; Rajesh Pandey; Viktoriya S. Tsancheva; Alessia Candeo; Stanley W. Botchway; Alasdair J. Allan; Lydia Teboul; Kamel Madi; Tahkur S. Babra; Laura A. K. Zolkiewski; Xiaojing Xue; Louise Bentley; John Gannon; Sam N. Olof; Roger D. Cox
Biofabrication, 12(1), 015018 (2020), DOI: 10.1088/1758-5090/ab56fe.
2019
Multiphoton fluorescence lifetime imaging microscopy (FLIM) and super-resolution fluorescence imaging with a supramolecular biopolymer for the controlled tagging of polysaccharides
Haobo Ge; Ferran Cortezon-Tamarit; Huan-Cai Wang; Adam C. Sedgwick; Robert L. Arrowsmith; Vincenzo Mirabello; Stanley W. Botchway; Tony D. James; Sofia I. Pascu
Nanoscale, 11(19), 9498–9507 (2019), DOI: 10.1039/C8NR10344E.
Directed Molecular Stacking for Engineered Fluorescent Three-Dimensional Reduced Graphene Oxide and Coronene Frameworks
Boyang Mao; Ferran Cortezon-Tamarit; Haobo Ge; Navaratnarajah Kuganathan; Vincenzo Mirabello; F. J. Palomares; G. Kociok-Köhn; Stanley W. Botchway; David G. Calatayud; Sofia I. Pascu
ChemistryOpen, 8(12), 1383–1398 (2019), DOI: 10.1002/open.201900310.
Super-resolution Microscopy at Cryogenic Temperatures Using Solid Immersion Lenses
Benji C. Bateman; Laura C. Zanetti-Domingues; Amy N. Moores; Sarah R. Needham; Daniel J. Rolfe; Lin Wang; David T. Clarke; Marisa L. Martin-Fernandez
Bio-protocol, 9(22), e3426 (2019), DOI: 10.21769/BioProtoc.3426.
A global sampler of single particle tracking solutions for single molecule microscopy
Michael Hirsch; Richard Wareham; Ji W. Yoon; Daniel J. Rolfe; Laura C. Zanetti-Domingues; Michael P. Hobson; Peter J. Parker; Marisa L. Martin-Fernandez; Sumeetpal S. Singh
PLOS ONE, 14(10), e0221865 (2019), DOI: 10.1371/journal.pone.0221865.
A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles
Javier Periz; Mario Del Rosario; Alexandra McStea; Simon Gras; Colin Loney; Lin Wang; Marisa L. Martin-Fernandez; Markus Meissner
Nature Communications, 10(1), 4183 (2019), DOI: 10.1038/s41467-019-12136-2.
The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis
James F. McKenna; Daniel J. Rolfe; Stephen E. D. Webb; Andrew F. Tolmie; Stanley W. Botchway; Marisa L. Martin-Fernandez; Chris Hawes; John Runions
Proceedings of the National Academy of Sciences of the United States of America, 116(26), 12857–12862 (2019), DOI: 10.1073/pnas.1819077116.
A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor
David T. Clarke; Marisa L. Martin-Fernandez
Methods and Protocols, 2(1), 12 (2019), DOI: 10.3390/mps2010012.
Preparation of polymer gold nanoparticle composites with tunable plasmon coupling and their application as SERS substrates
Samir A. A. Belhout; F. R. Baptista; S. J. Devereux; Anthony W. Parker; Andrew D. Ward; Susan J. Quinn
Nanoscale, 11(42), 19884–19894 (2019), DOI: 10.1039/C9NR05014K.
A signal motif retains Arabidopsis ER-α-mannosidase I in the cis-Golgi and prevents enhanced glycoprotein ERAD
Jürgen Schoberer; Julia König; Christina Veit; Ulrike Vavra; Eva Liebminger; Stanley W. Botchway; Friedrich Altmann; Verena Kriechbaumer; Chris Hawes; Richard Strasser
Nature Communications, 10(1), 3701 (2019), DOI: 10.1038/s41467-019-11686-9.
Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor
Jemima Ho; Xuexin Yang; Spyridoula-Angeliki Nikou; Nessim Kichik; Andrew Donkin; Nicole O. Ponde; Jonathan P. Richardson; Remi L. Gratacap; Linda S. Archambault; Christian P. Zwirner; Celia Murciano; Rhonda Henley-Smith; Selvam Thavaraj; Christopher J. Tynan; Sarah L. Gaffen; Bernhard Hube; Robert T. Wheeler; David L. Moyes; Julian R. Naglik
Nature Communications, 10(1), 2297 (2019), DOI: 10.1038/s41467-019-09915-2.
Manipulation and Deposition of Complex, Functional Block Copolymer Nanostructures Using Optical Tweezers
Olivia E. C. Gould; Stephen J. Box; Charlotte E. Boott; Andrew D. Ward; Mitchell A. Winnik; Mervyn J. Miles; Ian Manners
ACS Nano, 13(4), 3858–3866 (2019), DOI: 10.1021/acsnano.9b00342.
Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution
Lin Wang; Benji Bateman; Laura C. Zanetti-Domingues; Amy N. Moores; Sam Astbury; Christopher Spindloe; Michele C. Darrow; Maria Romano; Sarah R. Needham; Konstantinos Beis; Daniel J. Rolfe; David T. Clarke; Marisa L. Martin-Fernandez
Communications Biology, 2, 74 (2019), DOI: 10.1038/s42003-019-0317-6.
Structure and Dynamics of the EGF Receptor as Revealed by Experiments and Simulations and Its Relevance to Non-Small Cell Lung Cancer
Marisa L. Martin-Fernandez; David T. Clarke; Selene K. Roberts; Laura C. Zanetti-Domingues; Francesco L. Gervasio
Cells, 8(4), 316 (2019), DOI: 10.3390/cells8040316.
The Rho family GEF FARP2 is activated by aPKCι to control tight junction formation and polarity
Ahmed Elbediwy; Yixiao Zhang; Mathias Cobbaut; Philippe Riou; Ray S. Tan; Selene K. Roberts; Christopher J. Tynan; Roger George; Svend Kjaer; Marisa L. Martin-Fernandez; Barry J. Thompson; Neil Q. McDonald; Peter J. Parker
Journal of Cell Science, 132(8), jcs223743 (2019), DOI: 10.1242/jcs.223743.
The Golgi Localization of GnTI Requires a Polar Amino Acid Residue within Its Transmembrane Domain
Jürgen Schoberer; Eva Liebminger; Ulrike Vavra; Christina Veit; Christian Grünwald-Gruber; Friedrich Altmann; Stanley W. Botchway; Richard Strasser
Plant Physiology, 180(2), 859–873 (2019), DOI: 10.1104/pp.19.00310.
Ataxia-Telangiectasia Mutated is located in cardiac mitochondria and impacts oxidative phosphorylation
Marlene Blignaut; Ben Loos; Stanley W. Botchway; Anthony W. Parker; Barbara Huisamen
Scientific Reports, 9(1), 4782 (2019), DOI: 10.1038/s41598-019-41108-1.
DNA DSB Repair Dynamics following Irradiation with Laser-Driven Protons at Ultra-High Dose Rates
F. Hanton; P. Chaudhary; D. Doria; D. Gwynne; C. Maiorino; C. Scullion; H. Ahmed; T. Marshall; K. Naughton; L. Romagnani; S. Kar; G. Schettino; P. McKenna; Stanley W. Botchway; D. R. Symes; P. P. Rajeev; K. M. Prise; M. Borghesi
Scientific Reports, 9(1), 4471 (2019), DOI: 10.1038/s41598-019-40339-6.
Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells
Abdullah R. Ahmed; R. J. Owens; C. D. Stubbs; Anthony W. Parker; R. Hitchman; Rahul B. Yadav; M. Dumoux; Chris Hawes; Stanley W. Botchway
Scientific Reports, 9(1), 3408 (2019), DOI: 10.1038/s41598-019-39410-z.
Controlling the fluorescence and room-temperature phosphorescence behaviour of carbon nanodots with inorganic crystalline nanocomposites
David C. Green; Mark A. Holden; Mark A. Levenstein; Shuheng Zhang; Benjamin R. G. Johnson; Juan Gala de Pablo; Andrew D. Ward; Stanley W. Botchway; Fiona C. Meldrum
Nature Communications, 10(1), 206 (2019), DOI: 10.1038/s41467-018-08214-6.
2018
The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers
Laura C. Zanetti-Domingues; Dimitrios Korovesis; Sarah R. Needham; Christopher J. Tynan; Shiori Sagawa; Selene K. Roberts; Antonija Kuzmanic; Elena Ortiz-Zapater; Purvi Jain; Rob C. Roovers; Alireza Lajevardipour; Paul M. P. van Bergen en Henegouwen; George Santis; Andrew H. A. Clayton; David T. Clarke; Francesco L. Gervasio; Yibing Shan; David E. Shaw; Daniel J. Rolfe; Peter J. Parker; Marisa L. Martin-Fernandez
Nature Communications, 9(1), 4325 (2018), DOI: 10.1038/s41467-018-06632-0.
Cluster Analysis of Endogenous HER2 and HER3 Receptors in SKBR3 Cells
Selene K. Roberts; Michael Hirsch; Alexandra McStea; Laura C. Zanetti-Domingues; David T. Clarke; Jeroen Claus; Peter J. Parker; Lin Wang; Marisa L. Martin-Fernandez
Bio-protocol, 8(23), e3096 (2018), DOI: 10.21769/BioProtoc.3096.
Iridium Nanoparticles for Multichannel Luminescence Lifetime Imaging, Mapping Localization in Live Cancer Cells
S. M. King; S. Claire; R. I. Teixeira; A. N. Dosumu; A. J. Carrod; H. Dehghani; M. J. Hannon; Andrew D. Ward; R. Bicknell; Stanley W. Botchway; N. J. Hodges; Z. Pikramenou
Journal of the American Chemical Society, 140(32), 10242–10249 (2018), DOI: 10.1021/jacs.8b05105.
Synthesis, Radiolabelling and In Vitro Imaging of Multifunctional Nanoceramics
M. Lledos; V. Mirabello; S. Sarpaki; H. Ge; H. J. Smugowski; L. Carroll; E. O. Aboagye; F. I. Aigbirhio; Stanley W. Botchway; J. R. Dilworth; D. G. Calatayud; P. K. Plucinski; G. J. Price; S. I. Pascu
ChemNanoMat, 4(4), 361–372 (2018), DOI: 10.1002/cnma.201700378.
High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
Jason M. Cole; Daniel R. Symes; Nelson C. Lopes; Jonathan C. Wood; Kristjan Poder; Saleh Alatabi; Stanley W. Botchway; Peta S. Foster; Sarah Gratton; Sara Johnson; Christos Kamperidis; Olena Kononenko; Michael De Lazzari; Charlotte A. J. Palmer; Dean Rusby; James Sanderson; Matthew Sandholzer; Gianluca Sarri; Zsuzsanna Szoke-Kovacs; Lydia Teboul; J. M. Thompson; J. R. Warwick; H. Westerberg; Mark A. Hill; David P. Norris; Stuart P. D. Mangles; Zulfikar Najmudin
Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6335–6340 (2018), DOI: 10.1073/pnas.1802314115.
Super-Resolution Nanoscopy Imaging Applied to DNA Double-Strand Breaks
Sofia D'Abrantes; Sarah Gratton; Paul Reynolds; Verena Kriechbaumer; James McKenna; Stephen Barnard; David T. Clarke; Stanley W. Botchway
Radiation Research, 189(1), 19–31 (2018), DOI: 10.1667/RR14594.1.
Determination of the refractive index of insoluble organic extracts from atmospheric aerosol over the visible wavelength range using optical tweezers
Rosalie H. Shepherd; Martin D. King; Amelia A. Marks; Neil Brough; Andrew D. Ward
Atmospheric Chemistry and Physics, 18(8), 5235–5252 (2018), DOI: 10.5194/acp-18-5235-2018.
1064 nm Dispersive Raman Microspectroscopy and Optical Trapping of Pharmaceutical Aerosols
Peter J. Gallimore; Nick M. Davidson; Markus Kalberer; Francis D. Pope; Andrew D. Ward
Analytical Chemistry, 90(15), 8838–8844 (2018), DOI: 10.1021/acs.analchem.8b00817.
Inhibitor-induced HER2-HER3 heterodimerisation promotes proliferation through a novel dimer interface
Jeroen Claus; Gargi Patel; Flavia Autore; Audrey Colomba; Gregory Weitsman; Tanya N. Soliman; Selene K. Roberts; Laura C. Zanetti-Domingues; Michael Hirsch; Francesca Collu; Roger George; Elena Ortiz-Zapater; Paul R. Barber; Boris Vojnovic; Yosef Yarden; Marisa L. Martin-Fernandez; Angus Cameron; Franca Fraternali; Tony Ng; Peter J. Parker
eLife, 7, e32271 (2018), DOI: 10.7554/eLife.32271.
Dissecting single-cell molecular spatiotemporal mobility and clustering at focal adhesions in polarised cells by fluorescence fluctuation spectroscopy methods
Esther Garcia; Jorge Bernardino de la Serna
Methods, 140–141, 85–96 (2018), DOI: 10.1016/j.ymeth.2018.03.008.
The odd one out: Arabidopsis reticulon 20 does not bend ER membranes but has a role in lipid regulation
Verena Kriechbaumer; Lilly Maneta-Peyret; Laetitia Fouillen; Stanley W. Botchway; Jessica Upson; Louise Hughes; Jake Richardson; Maike Kittelmann; Patrick Moreau; Chris Hawes
Scientific Reports, 8(1), 2310 (2018), DOI: 10.1038/s41598-018-20840-0.
Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
Imogen Sparkes; Rhiannon R. White; Benjamin C. Coles; Stanley W. Botchway; Andrew D. Ward
The Plant Endoplasmic Reticulum: Methods and Protocols, Methods in Molecular Biology, Vol. 1691, pp. 167–178 (2018), DOI: 10.1007/978-1-4939-7389-7_13.
Metabolons on the Plant ER
Verena Kriechbaumer; Stanley W. Botchway
The Plant Endoplasmic Reticulum: Methods and Protocols, Methods in Molecular Biology, Vol. 1691, pp. 159–166 (2018), DOI: 10.1007/978-1-4939-7389-7_12.
High-Resolution Microscopy for Structural Studies of Biological Systems in Cells
David T. Clarke; Laura C. Zanetti-Domingues; Marisa L. Martin-Fernandez
eLS, pp. 1–10 (2018), DOI: 10.1002/9780470015902.a0027945.
2017
Affimer proteins are versatile and renewable affinity reagents
Christian Tiede, Robert Bedford, Sophie J. Heseltine, Gina Smith, Imeshi Wijetunga, Rebecca Ross, Danah AlQallaf, Ashley P. E. Roberts, Alexander Balls, Alistair Curd, Ruth E. Hughes, Heather Martin, Sarah R. Needham, Laura C. Zanetti-Domingues, Yashar Sadigh, Thomas P. Peacock, Anna A. Tang, Naomi Gibson, Hannah Kyle, Geoffrey W. Platt, Nicola Ingram, Thomas Taylor, Louise P. Coletta, Iain Manfield, Margaret Knowles, Sandra Bell, Filomena Esteves, Azhar Maqbool, Raj K. Prasad, Mark Drinkhill, Robin S. Bon, Vikesh Patel, Sarah A. Goodchild, Marisa Martin-Fernandez, Ray J. Owens, Joanne E. Nettleship, Michael E. Webb, Michael Harrison, Jonathan D. Lippiat, Sreenivasan Ponnambalam, Michelle Peckham, Alastair Smith, Paul K. Ferrigno, Matt Johnson, Michael J. McPherson, Darren C. Tomlinson
eLife, 6, e24903 (2017), DOI: 10.7554/eLife.24903.
Behavior of Supramolecular Assemblies of Radiometal-Filled and Fluorescent Carbon Nanocapsules In Vitro and In Vivo
Haobo Ge, Patrick J. Riss, Vincenzo Mirabello, David G. Calatayud, Stephen E. Flower, Rory L. Arrowsmith, Tim D. Fryer, Young Hong, Steve Sawiak, Robert M. J. Jacobs, Stanley W. Botchway, Rex M. Tyrrell, Tony D. James, John S. Fossey, Jonathan R. Dilworth, Franklin I. Aigbirhio, Sofia I. Pascu
Chem, 3(3), 437–460 (2017), DOI: 10.1016/j.chempr.2017.06.013.
Fluorescence-Lifetime Imaging and Super-Resolution Microscopies Shed Light on the Directed- and Self-Assembly of Functional Porphyrins onto Carbon Nanotubes and Flat Surfaces
Boyang Mao, David G. Calatayud, Vincenzo Mirabello, Navaratnarajah Kuganathan, Haobo Ge, Robert M. J. Jacobs, Ashley M. Shepherd, José A. Ribeiro Martins, Jorge Bernardino de la Serna, Benjamin J. Hodges, Stanley W. Botchway, Sofia I. Pascu
Chemistry – A European Journal, 23(41), 9772–9789 (2017), DOI: 10.1002/chem.201605232.
Lysosomal tracking with a cationic naphthalimide using multiphoton fluorescence lifetime imaging microscopy
Meng Li, Haobo Ge, Vincenzo Mirabello, Rory L. Arrowsmith, Gabriele Kociok-Köhn, Stanley W. Botchway, Weihong Zhu, Sofia I. Pascu, Tony D. James
Chemical Communications, 53(81), 11161–11164 (2017), DOI: 10.1039/C7CC05166B.
Multimodal Probes: Superresolution and Transmission Electron Microscopy Imaging of Mitochondria, and Oxygen Mapping of Cells, Using Small-Molecule Ir(III) Luminescent Complexes
Jonathan R. Shewring, Ahmet J. Cankut, Luke K. McKenzie, Bethany J. Crowston, Stanley W. Botchway, Julia A. Weinstein, Elizabeth Edwards, Michael D. Ward
Inorganic Chemistry, 56(24), 15259–15270 (2017), DOI: 10.1021/acs.inorgchem.7b02633.
Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria
Sreejesh Sreedharan, Martin R. Gill, Esther Garcia, Hiwa K. Saeed, Darren Robinson, Aisling Byrne, Ashley J. Cadby, Tia E. Keyes, Carl G. W. Smythe, Patrina Pellett, Jorge Bernardino de la Serna, Jim A. Thomas
Journal of the American Chemical Society, 139(44), 15907–15913 (2017), DOI: 10.1021/jacs.7b08772.
New Approaches to Photodynamic Therapy from Types I, II and III to Type IV Using One or More Photons
Kathrin M. Scherer, Roger H. Bisby, Stanley W. Botchway, Anthony W. Parker
Anti-Cancer Agents in Medicinal Chemistry, 17(2), 171–189 (2017), DOI: 10.2174/1871520616666160513131723.
Oxygen Mapping of Melanoma Spheroids using Small Molecule Platinum Probe and Phosphorescence Lifetime Imaging Microscopy
Ahmed Raza, Helen E. Colley, Elizabeth Baggaley, Igor V. Sazanovich, Nicola H. Green, Julia A. Weinstein, Stanley W. Botchway, Sheila MacNeil, John W. Haycock
Scientific Reports, 7(1), 10743 (2017), DOI: 10.1038/s41598-017-11153-9.
Stacks off tracks: a role for the golgin AtCASP in plant endoplasmic reticulum-Golgi apparatus tethering
Anne Osterrieder, Imogen A. Sparkes, Stanley W. Botchway, Andrew D. Ward, Tijs Ketelaar, Norbert C. A. de Ruijter, Chris Hawes
Journal of Experimental Botany, 68(13), 3339–3350 (2017), DOI: 10.1093/jxb/erx167.
Measurement of the Raman spectra and hygroscopicity of four pharmaceutical aerosols as they travel from pressurised metered dose inhalers (pMDI) to a model lung
Nick Davidson, Hai-Jie Tong, Markus Kalberer, Peter C. Seville, Andrew D. Ward, Marina K. Kuimova, Francis D. Pope
International Journal of Pharmaceutics, 520(1–2), 59–69 (2017), DOI: 10.1016/j.ijpharm.2017.01.051.
2016
Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters
Tess A. Stanly; Marco Fritzsche; Suneale Banerji; Esther García; Jorge Bernardino de la Serna; David G. Jackson; Christian Eggeling
Biology Open, 5(9), 1343–1350 (2016), DOI: 10.1242/bio.019943
WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion
Esther García; Chiara Ragazzini; Xia Yu; Elena Cuesta-García; Jorge Bernardino de la Serna; Tobias Zech; David Sarrió; Laura M. Machesky; Isabel M. Antón
Scientific Reports, 6, 23590 (2016), DOI: 10.1038/srep23590
EGFR oligomerization organizes kinase-active dimers into competent signalling platforms
Sarah R. Needham; Selene K. Roberts; Anton Arkhipov; Venkatesh P. Mysore; Christopher J. Tynan; Laura C. Zanetti-Domingues; Eric T. Kim; Valeria Losasso; Dimitrios Korovesis; Michael Hirsch; Daniel J. Rolfe; David T. Clarke; Martyn D. Winn; Alireza Lajevardipour; Andrew H. A. Clayton; Linda J. Pike; Michela Perani; Peter J. Parker; Yibing Shan; David E. Shaw; Marisa L. Martin-Fernandez
Nature Communications, 7, 13307 (2016), DOI: 10.1038/ncomms13307
Dynamic viscosity mapping of the oxidation of squalene aerosol particles
Athanasios Athanasiadis; Clare Fitzgerald; Nicholas M. Davidson; Chiara Giorio; Stanley W. Botchway; Andrew D. Ward; Markus Kalberer; Francis D. Pope; Marina K. Kuimova
Physical Chemistry Chemical Physics, 18(44), 30385–30393 (2016), DOI: 10.1039/C6CP05674A
A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon
Emily Breeze; Natasha Dzimitrowicz; Verena Kriechbaumer; R. Brooks; Stanley W. Botchway; J. P. Brady; Chris Hawes; A. M. Dixon; J. R. Schnell; M. D. Fricker; Lorenzo Frigerio
Proceedings of the National Academy of Sciences of the United States of America, 113(39), 10902–10907 (2016), DOI: 10.1073/pnas.1605434113
The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes
Ana Katrina Estandarte; Stanley W. Botchway; Christophe Lynch; Mohammed Yusuf; Ian Robinson
Scientific Reports, 6, 31417 (2016), DOI: 10.1038/srep31417
Fluorescence lifetime imaging of optically levitated aerosol: a technique to quantitatively map the viscosity of suspended aerosol particles
Clare Fitzgerald; Neveen A. Hosny; Haijie Tong; Peter C. Seville; Peter J. Gallimore; Nicholas M. Davidson; Athanasios Athanasiadis; Stanley W. Botchway; Andrew D. Ward; Markus Kalberer; Marina K. Kuimova; Francis D. Pope
Physical Chemistry Chemical Physics, 18(31), 21710–21719 (2016), DOI: 10.1039/C6CP03674K
Heteronuclear Ir(III)-Ln(III) Luminescent Complexes: Small-Molecule Probes for Dual Modal Imaging and Oxygen Sensing
Atanu Jana; Bethany J. Crowston; Jonathan R. Shewring; Luke K. McKenzie; Helen E. Bryant; Stanley W. Botchway; Andrew D. Ward; Angelo J. Amoroso; Elizabeth Baggaley; Michael D. Ward
Inorganic Chemistry, 55(11), 5623–5633 (2016), DOI: 10.1021/acs.inorgchem.6b00702
Molybdenum Speciation and its Impact on Catalytic Activity during Methane Dehydroaromatization in Zeolite ZSM-5 as Revealed by Operando X-Ray Methods
I. Lezcano-González; R. Oord; M. Rovezzi; P. Glatzel; Stanley W. Botchway; B. M. Weckhuysen; A. M. Beale
Angewandte Chemie International Edition, 55(17), 5215–5219 (2016), DOI: 10.1002/anie.201601357
Characterisation of the effects of optical aberrations in single molecule techniques
Benjamin C. Coles; Stephen E. D. Webb; Noah Schwartz; Daniel J. Rolfe; Marisa L. Martin-Fernandez; Valentina Lo Schiavo
Biomedical Optics Express, 7(5), 1755–1767 (2016), DOI: 10.1364/BOE.7.001755
A tale of the epidermal growth factor receptor: The quest for structural resolution on cells
Christopher J. Tynan; Valentina Lo Schiavo; Laura C. Zanetti-Domingues; Sarah R. Needham; Selene K. Roberts; Michael Hirsch; Daniel J. Rolfe; Dimitrios Korovesis; David T. Clarke; Marisa L. Martin-Fernandez
Methods, 95, 86–93 (2016), DOI: 10.1016/j.ymeth.2015.10.009
Imaging and radiation effects of gold nanoparticles in tumour cells
Hannah N. McQuaid; Mark F. Muir; Laura E. Taggart; Stephen J. McMahon; Jonathan A. Coulter; William B. Hyland; Suneil Jain; Karl T. Butterworth; Giuseppe Schettino; Kevin M. Prise; David G. Hirst; Stanley W. Botchway; Frederick J. Currell
Scientific Reports, 6, 19442 (2016), DOI: 10.1038/srep19442
Interactions Between an Aryl Thioacetate-Functionalized Zn(II) Porphyrin and Graphene Oxide
Boyang Mao; David G. Calatayud; Vincenzo Mirabello; Benjamin J. Hodges; José Alberto Martins; Stanley W. Botchway; John M. Mitchels; Sofia I. Pascu
Advanced Functional Materials, 26(5), 687–697 (2016), DOI: 10.1002/adfm.201504147
In Vivo Quantification of Peroxisome Tethering to Chloroplasts in Tobacco Epidermal Cells Using Optical Tweezers
Hongbo Gao; Jeremy Metz; Nicholas A. Teanby; Andrew D. Ward; Stanley W. Botchway; Benjamin C. Coles; Mark R. Pollard; Imogen Sparkes
Plant Physiology, 170(1), 263–272 (2016), DOI: 10.1104/pp.15.01529
Microwave gallium-68 radiochemistry for kinetically stable bis(thiosemicarbazone) complexes: structural investigations and cellular uptake under hypoxia
Imran S. Alam; Rory L. Arrowsmith; Fernando Cortezon-Tamarit; Frances Twyman; Gabriele Kociok-Köhn; Stanley W. Botchway; Jonathan R. Dilworth; Louis Carroll; Eric O. Aboagye; Sofia I. Pascu
Dalton Transactions, 45(1), 144–155 (2016), DOI: 10.1039/C5DT02537K
2015
Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe
M. Simons; M. R. Pollard; C. D. Hughes; A. D. Ward; B. Van Houten; M. Towrie; S. W. Botchway; A. W. Parker; N. M. Kad
Scientific Reports, 5, 18486 (2015), DOI: 10.1038/srep18486
Effect of phosphorylation on the EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM
Oana Coban; Laura Zanetti-Domingues; Daniel R. Matthews; Daniel J. Rolfe; Gregory Weitsman; Paul R. Barber; Jody Barbeau; Viviane Devauges; Florian Kampmeier; Martyn Winn; Borivoj Vojnovic; Peter J. Parker; Keith A. Lidke; Diane S. Lidke; Simon M. Ameer-Beg; Marisa L. Martin-Fernandez; Tony Ng
Biophysical Journal, 108(5), 1013–1026 (2015), DOI: 10.1016/j.bpj.2015.01.005
Nanometric molecular separation measurements by single molecule photobleaching
Stephen E. D. Webb; Michael Hirsch; Sarah R. Needham; Benjamin C. Coles; Kathrin M. Scherer; Selene K. Roberts; Laura C. Zanetti-Domingues; Christopher J. Tynan; Marisa L. Martin-Fernandez; Daniel J. Rolfe
Methods, 88, 76–80 (2015), DOI: 10.1016/j.ymeth.2015.05.009
Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation
Kathrin M. Scherer; Roger H. Bisby; Stanley W. Botchway; John A. Hadfield; John W. Haycock; Anthony W. Parker
Journal of Biomedical Optics, 20(7), 078003 (2015), DOI: 10.1117/1.JBO.20.7.078003
Reticulomics: Protein-protein interaction studies with two plasmodesmata-localised reticulon family proteins identify binding partners enriched at plasmodesmata, ER and the plasma membrane
Verena Kriechbaumer; Stanley W. Botchway; Susan E. Slade; Kirsten Knox; Lorenzo Frigerio; Karl J. Oparka; Chris Hawes
Plant Physiology, 169(3), 1933–1945 (2015), DOI: 10.1104/pp.15.01153
Environmental Pollutant Ozone Causes Damage to Lung Surfactant Protein B (SP-B)
J. M. Hemming; B. R. Hughes; A. R. Rennie; S. Tomas; R. A. Campbell; A. V. Hughes; T. Arnold; Stanley W. Botchway; K. C. Thompson
Biochemistry, 54(33), 5185–5197 (2015), DOI: 10.1021/acs.biochem.5b00308
Determining the geometry of oligomers of the human epidermal growth factor family on cells with 7 nm resolution
Laura C. Zanetti-Domingues; Michael Hirsch; Christopher J. Tynan; Daniel J. Rolfe; Teodor V. Boyadzhiev; Kathrin M. Scherer; David T. Clarke; Marisa L. Martin-Fernandez; Sarah R. Needham
Progress in Biophysics and Molecular Biology, 118(3), 139–152 (2015), DOI: 10.1016/j.pbiomolbio.2015.04.002
Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution
Sarah R. Needham; Laura C. Zanetti-Domingues; Kathrin M. Scherer; Michael Hirsch; Daniel J. Rolfe; Selene K. Roberts; Marisa L. Martin-Fernandez; David T. Clarke; Christopher J. Tynan
Biochemical Society Transactions, 43(3), 309–314 (2015), DOI: 10.1042/BST20140318
Imaging intracellular and systemic in vivo gold nanoparticles to enhance radiotherapy
Stanley W. Botchway; Jonathan A. Coulter; Frederick J. Currell
British Journal of Radiology, 88(1054), 20150170 (2015), DOI: 10.1259/bjr.20150170
A series of flexible design adaptations to the Nikon E-C1 and E-C2 confocal microscope systems for UV, multiphoton and FLIM imaging
Stanley W. Botchway; Kathrin M. Scherer; Steve Hook; Christopher D. Stubbs; Eleanor Weston; Roger H. Bisby; Anthony W. Parker
Journal of Microscopy, 258(1), 68–78 (2015), DOI: 10.1111/jmi.12218
A fluorescent Arg-Gly-Asp (RGD) peptide-naphthalenediimide (NDI) conjugate for imaging integrin αvβ3 in vitro
Zhiyuan Hu; Rory L. Arrowsmith; James A. Tyson; Vincenzo Mirabello; Haobo Ge; Ian M. Eggleston; Stanley W. Botchway; G. Dan Pantoş; Sofia I. Pascu
Chemical Communications, 51(32), 6901–6904 (2015), DOI: 10.1039/C4CC08265F
Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex
Rory L. Arrowsmith; Anthony J. Atkin; Stanley W. Botchway; Ian J. S. Fairlamb; Jason M. Lynam; James W. B. Moir; Sofia I. Pascu; Jonathan S. Ward; Wei-Qiang Zhang
Dalton Transactions, 44(11), 4957–4962 (2015), DOI: 10.1039/C4DT03312D
Anticancer phototherapy using activation of E-combretastatins by two-photon-induced isomerization
Kathrin M. Scherer; Roger H. Bisby; Stanley W. Botchway; John A. Hadfield; Anthony W. Parker
Journal of Biomedical Optics, 20(5), 051004 (2015), DOI: 10.1117/1.JBO.20.5.051004
Microfluidic generation of monodisperse ultra-low interfacial tension oil droplets in water
Guido Bolognesi; Alex Hargreaves; Andrew D. Ward; Andrew K. Kirby; Colin D. Bain; Oscar Ces
RSC Advances, 5(11), 8114–8121 (2015), DOI: 10.1039/C4RA14967J
Atmospherically relevant core-shell aerosol studied using optical trapping and Mie scattering
Stephanie H. Jones; Martin D. King; Andrew D. Ward
Chemical Communications, 51(23), 4914–4917 (2015), DOI: 10.1039/C4CC09835H
Heterogeneous oxidation of nitrite anion by ozone in aqueous droplets
Oliver R. Hunt; Andrew D. Ward; Martin D. King
Physical Chemistry Chemical Physics, 17(4), 2734–2741 (2015), DOI: 10.1039/C4CP05062B
Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems
Debbie L. Jones; Michael B. Andrews; Adam N. Swinburne; Stanley W. Botchway; Andrew D. Ward; Jonathan R. Lloyd; Louise S. Natrajan
Chemical Science, 6(9), 5133–5138 (2015), DOI: 10.1039/C5SC00661A
2014
Re and 99mTc complexes of BodP3 – multi-modality imaging probes
Laura H. Davies; Benjamin B. Kasten; Paul D. Benny; Rory L. Arrowsmith; Haobo Ge; Sofia I. Pascu; Stanley W. Botchway; William Clegg; Ross W. Harrington; Lee J. Higham
Chemical Communications, 50(98), 15503–15505 (2014), DOI: 10.1039/C4CC06367H
The transmembrane domain of N-acetylglucosaminyltransferase I is the key determinant for its Golgi subcompartmentation
Jennifer Schoberer; Eva Liebminger; Ulrike Vavra; Christiane Veit; Alexandra Castilho; Martina Dicker; Daniel Maresch; Friedrich Altmann; Chris Hawes; Stanley W. Botchway; Richard Strasser
The Plant Journal, 80(5), 809–822 (2014), DOI: 10.1111/tpj.12671
Combined two-photon excitation and d→f energy transfer in a water-soluble Ir(III)/Eu(III) dyad: two luminescence components from one molecule for cellular imaging
Elizabeth Baggaley; D. K. Cao; David Sykes; Stanley W. Botchway; Julia A. Weinstein; Michael D. Ward
Chemistry – A European Journal, 20(29), 8898–8903 (2014), DOI: 10.1002/chem.201403618
Ditopic boronic acid and imine-based naphthalimide fluorescence sensor for copper(II)
Meng Li; Haobo Ge; Rory L. Arrowsmith; Vincenzo Mirabello; Stanley W. Botchway; Weihong Zhu; Sofia I. Pascu; Tony D. James
Chemical Communications, 50(80), 11806–11809 (2014), DOI: 10.1039/C4CC03453H
Fluorescence lifetime imaging microscopy analysis of defects in multi-tube physical vapor transport grown Cd1-xZnxTe
Andreas Schneider; Matthew C. Veale; Steven J. Bell; Diana D. Duarte; Matthew D. Wilson; Paul Seller; Stanley W. Botchway; Ashutosh Choubey; Douglas Halliday
Physica Status Solidi A, 211(9), 2121–2125 (2014), DOI: 10.1002/pssa.201300724
Hydrothermal conversion of one-photon-fluorescent poly(4-vinylpyridine) into two-photon-fluorescent carbon nanodots
Katherine Lawrence; Fengjie Xia; Rory L. Arrowsmith; Haobo Ge; Geoffrey W. Nelson; John S. Foord; Monica Felipe-Sotelo; Nick D. M. Evans; John M. Mitchels; Stephen E. Flower; Stanley W. Botchway; Daniel Wolverson; Gazi N. Aliev; Tony D. James; Sofia I. Pascu; Frank Marken
Langmuir, 30(39), 11746–11752 (2014), DOI: 10.1021/la404866s
Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA
Elizabeth Baggaley; Martin R. Gill; Nicola H. Green; David Turton; Igor V. Sazanovich; Stanley W. Botchway; Carl Smythe; John W. Haycock; Julia A. Weinstein; Jim A. Thomas
Angewandte Chemie International Edition, 53(13), 3367–3371 (2014), DOI: 10.1002/anie.201309427
The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility
Tai Kiuchi; Elena Ortiz-Zapater; James Monypenny; Daniel R. Matthews; Jody Barbeau; Oana Coban; Katherine Lawler; Brian Burford; Daniel J. Rolfe; Emanuele de Rinaldis; Dimitra Dafou; Michael A. Simpson; Natalie Woodman; Sarah Pinder; Cheryl E. Gillett; Viviane Devauges; Simon P. Poland; Gilbert Fruhwirth; Pierfrancesco Marra; Ykelien L. Boersma; Andreas Plückthun; William J. Gullick; Yosef Yarden; George Santis; Martyn Winn; Marisa L. Martin-Fernandez; Peter Parker; Andrew Tutt; Simon M. Ameer-Beg; Tony Ng
Science Signaling, 7(339), ra78 (2014), DOI: 10.1126/scisignal.2005157
Heterogeneous Interaction of SiO2 with N2O5: Aerosol Flow Tube and Single Particle Optical Levitation-Raman Spectroscopy Studies
Mingjin Tang; J. C. J. Camp; L. Rkiouak; J. McGregor; I. M. Watson; R. A. Cox; M. Kalberer; Andrew D. Ward; F. D. Pope
Journal of Physical Chemistry A, 118(38), 8817–8827 (2014), DOI: 10.1021/jp506753c
Rapid interrogation of physical and chemical characteristics of salbutamol sulphate aerosol from a pMDI
H.-J. Tong; Clare Fitzgerald; P. J. Gallimore; M. Kalberer; M. K. Kuimova; P. C. Seville; Andrew D. Ward; F. D. Pope
Chemical Communications, 50(98), 15499–15502 (2014), DOI: 10.1039/C4CC05803H
Structure-function relationships and supramolecular organization of the EGFR on the cell surface
Sarah R. Needham; Laura C. Zanetti-Domingues; Michael Hirsch; Daniel J. Rolfe; Christopher J. Tynan; Selene K. Roberts; Marisa L. Martin-Fernandez; David T. Clarke
Biochemical Society Transactions, 42(1), 114–119 (2014), DOI: 10.1042/BST20130236
Long-lived metal complexes open up microsecond lifetime imaging microscopy under multiphoton excitation: from FLIM to PLIM and beyond
Elizabeth Baggaley; Stanley W. Botchway; John W. Haycock; Hayley Morris; Igor V. Sazanovich; J. A. Gareth Williams; Julia A. Weinstein
Chemical Science, 5(3), 879–886 (2014), DOI: 10.1039/C3SC51875B
Optical trapping and Raman spectroscopy of solid particles
L. Rkiouak; M. J. Tang; J. C. J. Camp; J. McGregor; I. M. Watson; R. A. Cox; M. Kalberer; Andrew D. Ward; F. D. Pope
Physical Chemistry Chemical Physics, 16(23), 11426–11434 (2014), DOI: 10.1039/C4CP00994K
Two-photon phosphorescence lifetime imaging of cells and tissues using a long-lived cyclometallated Npyridyl^Cphenyl^Npyridyl Pt(II) complex
Elizabeth Baggaley; Igor V. Sazanovich; J. A. Gareth Williams; John W. Haycock; Stanley W. Botchway; Julia A. Weinstein
RSC Advances, 4(66), 35003–35008 (2014), DOI: 10.1039/C4RA04489D
Single-Cell Raman Sorting
Mengqiu Li; Dan G. Boardman; Andrew D. Ward; Wei E. Huang
Environmental Microbiology: Methods and Protocols, Methods in Molecular Biology, Vol. 1096, pp. 147–153 (2014), DOI: 10.1007/978-1-62703-712-9_12.
Investigating Protein–Protein Interactions in the Plant Endomembrane System Using Multiphoton-Induced FRET-FLIM
Jennifer Schoberer; Stanley W. Botchway
Plant Endosomes: Methods and Protocols, Methods in Molecular Biology, Vol. 1209, pp. 81–95 (2014), DOI: 10.1007/978-1-4939-1420-3_6.
2013
A ‘pocket guide’ to total internal reflection fluorescence
Marisa L. Martin-Fernandez; Christopher J. Tynan; Stephen E. D. Webb
Journal of Microscopy, 252(1), 16–22 (2013), DOI: 10.1111/jmi.12070
Microcrystal manipulation with laser tweezers
Armin Wagner; Ramona Duman; Bob Stevens; Andrew D. Ward
Acta Crystallographica Section D: Biological Crystallography, 69(7), 1297–1302 (2013), DOI: 10.1107/S090744491300958X
Spatiotemporal dynamics of DNA repair proteins following laser microbeam induced DNA damage – When is a DSB not a DSB?
Pamela Reynolds; Stanley W. Botchway; Anthony W. Parker; Peter O’Neill
Mutation Research – Genetic Toxicology and Environmental Mutagenesis, 756(1–2), 14–20 (2013), DOI: 10.1016/j.mrgentox.2013.05.006
Laser heating of sulfuric acid droplets held in air by laser Raman tweezers
Oliver R. Hunt; Andrew D. Ward; Martin D. King
RSC Advances, 3, 19448–19454 (2013), DOI: 10.1039/C3RA44018D
Determining the unique refractive index properties of solid polystyrene aerosol using broadband Mie scattering from optically trapped beads
Stephanie H. Jones; Martin D. King; Andrew D. Ward
Physical Chemistry Chemical Physics, 15, 20735–20741 (2013), DOI: 10.1039/C3CP53498G
Second-harmonic scattering in aqueous urea solutions: evidence for solute clusters?
Martin R. Ward; Stanley W. Botchway; Andrew D. Ward; Andrew J. Alexander
Faraday Discussions, 167, 441–454 (2013), DOI: 10.1039/C3FD00089C
Time-Resolved Fluorescence Imaging Reveals Differential Interactions of N-Glycan Processing Enzymes across the Golgi Stack in Planta
Jennifer Schoberer; Eva Liebminger; Stanley W. Botchway; Richard Strasser; Chris Hawes
Plant Physiology, 161(4), 1737–1754 (2013), DOI: 10.1104/pp.112.210757
Degradation and Rearrangement of a Lung Surfactant Lipid at the Air-Water Interface during Exposure to the Pollutant Gas Ozone
Katherine C. Thompson; Stephanie H. Jones; Adrian R. Rennie; Martin D. King; Andrew D. Ward; Brian R. Hughes; Claire O. M. Lucas; Richard A. Campbell; Arwel V. Hughes
Langmuir, 29(14), 4594–4602 (2013), DOI: 10.1021/la304312y
Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding
Laura C. Zanetti-Domingues; Christopher J. Tynan; Daniel J. Rolfe; David T. Clarke; Marisa L. Martin-Fernandez
PLOS ONE, 8(9), e74200 (2013), DOI: 10.1371/journal.pone.0074200
Measuring EGFR Separations on Cells with ~10 nm Resolution via Fluorophore Localization Imaging with Photobleaching
Sarah R. Needham; Michael Hirsch; Daniel J. Rolfe; David T. Clarke; Laura C. Zanetti-Domingues; Richard Wareham; Marisa L. Martin-Fernandez
PLOS ONE, 8(5), e62331 (2013), DOI: 10.1371/journal.pone.0062331
A Stochastic Model for Electron Multiplication Charge-Coupled Devices – From Theory to Practice
Michael Hirsch; Richard J. Wareham; Marisa L. Martin-Fernandez; Michael P. Hobson; Daniel J. Rolfe
PLOS ONE, 8(1), e53671 (2013), DOI: 10.1371/journal.pone.0053671
Shining light on stability: cellular phototoxicity and stability of metal thiosemicarbazonate complexes
Philip A. Waghorn; Michael W. Jones; Mark B. M. Theobald; Rory L. Arrowsmith; Sofia I. Pascu; Stanley W. Botchway; Stephen Faulkner; Jonathan R. Dilworth
Chemical Science, 4(4), 1430–1441 (2013), DOI: 10.1039/C2SC21489J
mTOR direct interactions with Rheb-GTPase and raptor: sub-cellular localization using fluorescence lifetime imaging
Rahul B. Yadav; Pierre Burgos; Anthony W. Parker; Valentina Iadevaia; Christopher G. Proud; Rodger A. Allen; James P. O’Connell; Ananya Jeshtadi; Christopher D. Stubbs; Stanley W. Botchway
BMC Cell Biology, 14, 3 (2013), DOI: 10.1186/1471-2121-14-3
2012
A Systematic Investigation of Differential Effects of Cell Culture Substrates on the Extent of Artifacts in Single-Molecule Tracking
Laura C. Zanetti-Domingues; Marisa L. Martin-Fernandez; Sarah R. Needham; Daniel J. Rolfe; David T. Clarke
PLOS ONE, 7(9), e45655 (2012), DOI: 10.1371/journal.pone.0045655
Single Molecule Fluorescence Detection and Tracking in Mammalian Cells: The State-of-the Art and Future Perspectives
Marisa L. Martin-Fernandez; David T. Clarke
International Journal of Molecular Sciences, 13(11), 14742–14765 (2012), DOI: 10.3390/ijms131114742
Cell wall constrains lateral diffusion of plant plasma-membrane proteins
Alexandre Martinière; Irene Lavagi; Gayathri Nageswaran; Daniel J. Rolfe; Lilly Maneta-Peyret; Doan-Trung Luu; Stanley W. Botchway; Stephen E. D. Webb; Sébastien Mongrand; Christophe Maurel; Marisa L. Martin-Fernandez; Jürgen Kleine-Vehn; Jiří Friml; Patrick Moreau; John Runions
Proceedings of the National Academy of Sciences of the United States of America, 109(31), 12805–12810 (2012), DOI: 10.1073/pnas.1202040109
Multicolour Single Molecule Imaging in Cells with Near Infra-Red Dyes
Christopher J. Tynan; David T. Clarke; Benjamin C. Coles; Daniel J. Rolfe; Marisa L. Martin-Fernandez; Stephen E. D. Webb
PLOS ONE, 7(4), e36265 (2012), DOI: 10.1371/journal.pone.0036265
Multicolour single molecule imaging on cells using a supercontinuum source
Stephen E. D. Webb; Laura C. Zanetti-Domingues; Benjamin C. Coles; Daniel J. Rolfe; Richard J. Wareham; Marisa L. Martin-Fernandez
Biomedical Optics Express, 3(3), 400–406 (2012), DOI: 10.1364/BOE.3.000400
Direct interaction of baculovirus capsid proteins VP39 and EXON0 with kinesin-1 in insect cells determined by FRET-FLIM
John O. Danquah; Stanley W. Botchway; Ananya Jeshtadi; Linda A. King
Journal of Virology, 86(2), 844–853 (2012), DOI: 10.1128/JVI.06109-11
Incandescent porous carbon microspheres to light up cells: solution phenomena and cellular uptake
Paul Duffy; Luis M. Magno; Rahul B. Yadav; Selene K. Roberts; Andrew D. Ward; Stanley W. Botchway; Paul E. Colavita; Susan J. Quinn
Journal of Materials Chemistry, 22(2), 432–439 (2012), DOI: 10.1039/C1JM14303D
Human epidermal growth factor receptor (HER1) aligned on the plasma membrane adopts key features of Drosophila EGFR asymmetry
Marisa L. Martin-Fernandez
Biochemical Society Transactions, 40(1), 184–188 (2012), DOI: 10.1042/BST20110692
Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy
Selene K. Roberts; Christopher J. Tynan; Martyn Winn; Marisa L. Martin-Fernandez
Biochemical Society Transactions, 40(1), 189–194 (2012), DOI: 10.1042/BST20110632
Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols
Irene Mueller-Harvey; Walter Feucht; Jürgen Polster; Lucie Trnková; Pierre Burgos; Anthony W. Parker; Stanley W. Botchway
Analytica Chimica Acta, 719, 68–75 (2012), DOI: 10.1016/j.aca.2011.12.068
Interactions between amino acid-tagged naphthalenediimide and single walled carbon nanotubes for design/construction of new bioimaging probes
Zhiyuan Hu; G. Dan Pantoş; Navaratnarajah Kuganathan; Rory Arrowsmith; Robert M. J. Jacobs; Gabriele Kociok-Köhn; Justin O’Byrne; Kerstin Jurkschat; Pierre Burgos; Rex M. Tyrrell; Stanley W. Botchway; Jeremy K. M. Sanders; Sofia I. Pascu
Advanced Functional Materials, 22(3), 503–518 (2012), DOI: 10.1002/adfm.201101932
Laser-Induced Radiation Microbeam Technology and Simultaneous Real-Time Fluorescence Imaging in Live Cells
Stanley W. Botchway; Pamela Reynolds; Anthony W. Parker; Peter O'Neill
Methods in Enzymology: Imaging and Spectroscopic Analysis of Living Cells, Vol. 504, pp. 3–28 (2012), DOI: 10.1016/B978-0-12-391857-4.00001-X.
Investigating the Conformation of HER Membrane Proteins in Cells via Single Molecule and FLIM Microscopy
Marisa L. Martin-Fernandez; David T. Clarke; Michael Hirsch; Sarah R. Needham; Selene K. Roberts; Daniel J. Rolfe; Chris J. Tynan; Stephen E. D. Webb; Martyn Winn; Laura Zanetti Domingues
Molecular Imaging, Chapter 4, pp. 71–94 (2012), DOI: 10.5772/30192.
2011
Subcellular and single-molecule imaging of plant fluorescent proteins using TIRFM
Gema Vizcay-Barrena; Stephen E. D. Webb; Marisa L. Martin-Fernandez; Zoe A. Wilson
Journal of Experimental Botany, 62(15), 5419–5428 (2011), DOI: 10.1093/jxb/err212
Human epidermal growth factor receptor (EGFR) aligned on plasma membrane adopts key features of Drosophila EGFR asymmetry
Christopher J. Tynan; Selene K. Roberts; Daniel J. Rolfe; David T. Clarke; Harald H. Loeffler; Johannes Kästner; Martyn D. Winn; Peter J. Parker; Marisa L. Martin-Fernandez
Molecular and Cellular Biology, 31(11), 2241–2252 (2011), DOI: 10.1128/MCB.01431-10
CAR modulates E-cadherin dynamics in the presence of adenovirus type 5
F. Hussain; P. E. Morton; M. Snippe; J. Sullivan; C. Farmer; Marisa L. Martin-Fernandez; M. Parsons; G. Santis
PLOS ONE, 6(8), e23056 (2011), DOI: 10.1371/journal.pone.0023056
Development of fluorophore dynamics imaging as a probe for lipid domains in model vesicles and cell membranes
Stanley W. Botchway; Amanda M. Lewis; Christopher D. Stubbs
European Biophysics Journal, 40(2), 131–141 (2011), DOI: 10.1007/s00249-010-0631-x
Automated multidimensional single molecule fluorescence microscopy feature detection and tracking
Daniel J. Rolfe; Colin I. McLachlan; Michael Hirsch; Sarah R. Needham; Christopher J. Tynan; Stephen E. D. Webb; Marisa L. Martin-Fernandez; Michael P. Hobson
European Biophysics Journal, 40(10), 1167–1186 (2011), DOI: 10.1007/s00249-011-0747-7
Optics clustered to output unique solutions: A multi-laser facility for combined single molecule and ensemble microscopy
David T. Clarke; Stanley W. Botchway; Benjamin C. Coles; Sarah R. Needham; Selene K. Roberts; Daniel J. Rolfe; Christopher J. Tynan; Andrew D. Ward; Stephen E. D. Webb; Rahul Yadav; Laura Zanetti-Domingues; Marisa L. Martin-Fernandez
Review of Scientific Instruments, 82(9), 093705 (2011), DOI: 10.1063/1.3635536
Nanofluidic networks created and controlled by light
David A. Woods; C. D. Mellor; J. M. Taylor; Colin D. Bain; Andrew D. Ward
Soft Matter, 7(6), 2517–2520 (2011), DOI: 10.1039/C0SM01183E
2010
One and two photon fluorescent complexes of rhenium and their technetium analogues
Stanley W. Botchway; Jonathan R. Dilworth; Maria Salichou
Dalton Transactions, 39(22), 5219–5220 (2010), DOI: 10.1039/B927506A
Interaction of poxvirus intracellular mature virion proteins with the TPR domain of kinesin light chain in live infected cells revealed by two-photon-induced FRET-FLIM
Ananya Jeshtadi; Pierre Burgos; Christopher D. Stubbs; Anthony W. Parker; Linda A. King; Michael A. Skinner; Stanley W. Botchway
Journal of Virology, 84(24), 12886–12894 (2010), DOI: 10.1128/JVI.01395-10
Use of near infrared femtosecond lasers as sub-micron radiation microbeam for cell DNA damage and repair studies
Stanley W. Botchway; Pamela Reynolds; Anthony W. Parker; Peter O’Neill
Mutation Research – Reviews in Mutation Research, 704(1–3), 38–44 (2010), DOI: 10.1016/j.mrrev.2010.01.003
Optically trapped probes with nanometer-scale tips for femto-Newton force measurement
Mark R. Pollard; Stanley W. Botchway; Boris Chichkov; Emily Freeman; Roger N. J. Halsall; David W. K. Jenkins; Ian Loader; Aleksandr Ovsianikov; Anthony W. Parker; Robert Stevens; Renato Turchetta; Andrew D. Ward; Mike Towrie
New Journal of Physics, 12(11), 113056 (2010), DOI: 10.1088/1367-2630/12/11/113056
Five Arabidopsis reticulon isoforms share endoplasmic reticulum location, topology, and membrane-shaping properties
Imogen Sparkes; Nicholas Tolley; Isabel Aller; Julia Svozil; Anne Osterrieder; Stanley W. Botchway; Christopher Mueller; Lorenzo Frigerio; Chris Hawes
The Plant Cell, 22(4), 1333–1343 (2010), DOI: 10.1105/tpc.110.074385
Focal adhesions are sites of integrin extension
Janet A. Askari; Christopher J. Tynan; Stephen E. D. Webb; Marisa L. Martin-Fernandez; Christoph Ballestrem; Martin J. Humphries
Journal of Cell Biology, 188(6), 891–903 (2010), DOI: 10.1083/jcb.200907174
Self-association of Calcium-binding Protein S100A4 and Metastasis
T. M. Ismail; S. Zhang; D. G. Fernig; S. Gross; Marisa L. Martin-Fernandez; V. See; K. Tozawa; Christopher J. Tynan; G. Z. Wang; M. C. Wilkinson; P. S. Rudland; R. Barraclough
Journal of Biological Chemistry, 285(2), 914–922 (2010), DOI: 10.1074/jbc.M109.010892
Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging
Roger H. Bisby; Stanley W. Botchway; A. G. Crisostomo; J. Karolin; Anthony W. Parker; L. Schroder
Spectroscopy, 24(1–2), 137–142 (2010), DOI: 10.3233/SPE-2010-0415
Single molecule fluorescence imaging of receptor interactions in mammalian cell signalling
David T. Clarke; Daniel Rolfe; Stephen E. D. Webb; Martyn Winn; Marisa L. Martin-Fernandez
Microscopy: Science, Technology, Applications and Education, Vol. 1, pp. 737–746 (2010), DOI: no DOI assigned.
